Well anyways, I've finally decided to write about a test to detect anti-Mycoplasma pneumoniae antibodies, using SERODIA-MYCO II (also known as MPA), since now I'm currently in serology department. Alot of manual tests, if I may say.
First of all, Mycoplasma pneumoniae is a very small kind of bacterium that is usually associated with upper respiratory tract infections, together with fever, headaches, cough and malaise. It may lead to tracheobronchitis, which is a common respiratory infection characterized by inflammation of the trachea and bronchi. Its mode of transmission is person-to-person transmission by direct contact with the infected respiratory secretions, and it will usually take about 1-4 weeks before the symptoms will develop.
Principle
SERODIA-MYCO II is used in the detection of antibodies to Mycoplasma pneumoniae. It uses gelatin particles which are sensitized with extracted cell membrane components of Mycoplasma pneumoniae. Serum containing specific antibodies will react with the sensitized couloured gekatin particles to form a smooth mat of agglutinated particles in the microtitration tray.
Reagents
SERODIA-MYCO II kit, which includes:
- Sensitized particles
- Unsensitized partiles
- Positive control
- Sample diluent
Procedures
6 wells are required for a patient's sample
4 wells are required for control
- Label the wells (ID number or Control)
- Add 100ul of sample diluent to the first wells
- Add 25ul of sample diluent to the rest of the wells
- Add 25ul of positive control to the first well and mix well
- Bring 25ul of the mixed solution to the next well and mix well
- Repeat Step 5 until the last well, where 25ul will be discarded after mixing
- Repeat Steps 4-6 with patient's sample
- Add one drop of unsensitized particles into the second wells
- Add one drop of sensitized psricles into the rest of the wells starting from well 3
- Tap at the sides of the microtitration plate gentle to ensure proper mixing
- Cover the plate and incubate at room temperature
- Results to be read 3 hours later
Interpretation
Negative (-):
Definite compact button in the center of well with smooth round outer margin
Positive (+):
Definite large ring with firmly agglutinated particles spread within the circle
Positive (++):
Agglutinated particles spread out to cover th bottom of the well entirely
MAYAFIRHANA
TG02
RSS Feed (xml)
8 comments:
hi there =D
juz to confirm.....is this SERODIA-MYCO II test confirmatory? the 6 wells used for each patient sample is for measuring the titre of antibodies issit? the unsensitised particles served as negative control issit?
Xin Ni
TG02
Group 9
Hey, im guessing you are amir. Sorry if your not.
Niways, u mentioned about "Serum containing specific antibodies will react with the sensitized couloured gekatin particles to form a smooth mat of agglutinated particles in the microtitration tray"..why do serum react with gelatin particles?What actually triggers the reaction?
Dyana
0605169B
hey,
why is it that you need to use 6 wells for each patient and that for the gelatin particles to react with the antibodies in the body, i presume it to be the acting antigen. Am I correct?
Debbie
TG02
to xin ni
yeap yeap. its a confirmatory test, not screening test. and yes, its to measure the titre of antibodies, meaning how 'strong' the infection is. and final yeah, the unsensitised is the negative control =D
hey dy
sori sori. i fergot to include my name. im MYA by the way. haha
technically, the test is to test for the antibodies against Mycoplasma pneumoniae. so is there is a reaction, its due to the reaction between the antibodies present in the serum, and the gelatin particles. coz the gelatin particles contains the cell membrane components of the M.pneumoniae. so its like an antigen-antibody reaction.
hope it answers ur question =)
hey debbie
since this is titration and the sixth well represents 1:320, im guessing they think that value is anough to measure the titre of the antibodies.
if the sixth plate still shows a ++ agglutination, they will just report the value as >1:320.
the gelatin particles does consist of the cell membrane components of the M.pneumoniae. so, when it agglutinates, the reaction is very similiar to the antigen-antibody reaction.so yeah, i guess the gelatin does consist of the acting antigen.
Please do not use the green coloe. Can't see very well. Ms Chew old already. Eye-sight got problem.
Post a Comment